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您現(xiàn)在的位置:首頁(yè)  >  技術(shù)文章  >  min6| min6細(xì)胞株 支持STR鑒定

min6| min6細(xì)胞株 支持STR鑒定

發(fā)布時(shí)間:2017-05-25瀏覽:1831次

min6| min6細(xì)胞株 支持STR鑒定

 

產(chǎn)品名稱:min6細(xì)胞

種屬 :小鼠胰島β細(xì)胞株

生長(zhǎng)特性:貼壁

形態(tài) :上皮樣

培養(yǎng)基 :DMEM high glucose + 10%FBS+0.05 mM巰基乙醇

生長(zhǎng)條件: 95%空氣+5%二氧化碳 37攝氏度

凍存條件: 90%FBS+10%DMSO慧穎生物是一家專門(mén)從事細(xì)胞培養(yǎng)相關(guān)試劑公司,擁有完善細(xì)胞庫(kù)管理體系,公司4000多株細(xì)胞逐一做STR鑒定,為每個(gè)科研用戶負(fù)責(zé),提供無(wú)交叉污染,真實(shí),來(lái)源可靠的細(xì)胞株!

 

min6| min6細(xì)胞株 支持STR鑒定常見(jiàn)抗生素的配方:

氨芐青霉素

 

氨芐青霉素(ampicillin)(100mg/ml)縮寫(xiě):Amp

溶解1g氨芐青霉素鈉鹽于足量的水中,zui后定容至10ml。分裝成小份于-20℃貯存。常以25ug/ml~50ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。

 

羧芐青霉素

 

羧芐青霉素(carbenicillin)(50mg/ml)縮寫(xiě):Cab

溶解0.5g羧芐青霉素二鈉鹽于足量的水中,zui后定容至10ml。分裝成小份于-20℃貯存。常以25ug/ml~50ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。

 

甲氧西林

 

甲氧西林(methicillin)(100mg/ml)

溶解1g甲氧西林鈉于足量的水中,zui后定容至10ml。分裝成小份于-20℃貯存。常以37.5ug/ml終濃度與100ug/ml氨芐青霉素一起添加于生長(zhǎng)培養(yǎng)基。

 

卡那霉素

 

卡那霉素(kanamycin)(10mg/ml)縮寫(xiě):Kan

溶解100mg卡那霉素于足量的水中,zui后定容至10ml。分裝成小份于-20℃貯存。常以10ug/ml~50ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。

氯霉素

 

氯霉素(chloramphenicol)(25mg/ml)縮寫(xiě):Cm

溶解250mg氯霉素足量的無(wú)水乙醇中,zui后定容至10ml。分裝成小份于-20℃貯存。常以12.5ug/ml~25ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。

生物實(shí)驗(yàn)高清視頻教程

 

鏈霉素

 

鏈霉素(streptomycin)(50mg/ml)縮寫(xiě):Sm

溶解0.5g鏈霉素硫酸鹽于足量的無(wú)水乙醇中,zui后定容至10ml。分裝成小份于-20℃貯存。常以10ug/ml~50ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。

 

萘啶酮酸

 

萘啶酮酸(nalidixic acid)(5mg/ml)

溶解50mg萘啶酮酸鈉鹽于足量的水中,zui后定容至10ml。分裝成小份于-20℃貯存。常以15ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。

 

四環(huán)素

 

四環(huán)素(tetracyyline)(10mg/ml)縮寫(xiě):Tet

溶解100mg四環(huán)素鹽酸鹽于足量的水中,或者將無(wú)堿的四環(huán)素溶于無(wú)水乙醇,定容至10ml。分裝成小份用鋁箔包裹裝液管以免溶液見(jiàn)光,于-20℃貯存。常以10ug/ml~50ug/ml的終濃度添加于生長(zhǎng)培養(yǎng)基。(注:鎂離子是四環(huán)素的拮抗劑,四環(huán)素抗性菌的篩選應(yīng)使用不含鎂鹽的培養(yǎng)基,如LB培養(yǎng)基。)

 

紅霉素

 

紅霉素(erythromycin)(20mg/ml)縮寫(xiě):Em

容積為95%乙醇或冰醋酸,用錫箔包裹以免溶液見(jiàn)光,于-20℃貯存,稀釋劑為蒸餾水。

(紅霉素和四環(huán)素等在偏酸環(huán)境下抗菌活性是會(huì)下降的,而在偏堿環(huán)境則會(huì)增加,阿米卡星和頭孢類等在偏堿環(huán)境抗菌活性會(huì)下降,在偏酸環(huán)境是會(huì)增加。)

 

min6| min6細(xì)胞株 支持STR鑒定

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HK

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HREC

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HRPEpiC

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Human Retinal Pigment Epithelial Cells

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HLEpiC

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Human Lens Epithelial Cells

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HIPEpiC

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HConF

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HTMC

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HOCF

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Human Myometrial Microvascular Endothelial Cells

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HEMEC

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HAMSC

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HCMSC

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Human Chorionic Mesenchymal Stromal Cells

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HMSC-bm

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HMSC-ad

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MMenC

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MN-dsc

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MN-sc

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MA-c

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MM

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MMa-bm

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MLF

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MRMC

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MHSteC

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Mouse Hepatic Slate Cells

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MCM

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MCF

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MMSC-bm

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Mouse Mesenchymal Stem Cells-bone marrow

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MEF

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Mouse Embryonic Fibroblasts

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MEF-mt

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